全文获取类型
收费全文 | 1899篇 |
免费 | 131篇 |
国内免费 | 1篇 |
出版年
2023年 | 14篇 |
2022年 | 16篇 |
2021年 | 39篇 |
2020年 | 44篇 |
2019年 | 57篇 |
2018年 | 76篇 |
2017年 | 57篇 |
2016年 | 81篇 |
2015年 | 121篇 |
2014年 | 111篇 |
2013年 | 119篇 |
2012年 | 160篇 |
2011年 | 154篇 |
2010年 | 86篇 |
2009年 | 83篇 |
2008年 | 104篇 |
2007年 | 82篇 |
2006年 | 99篇 |
2005年 | 53篇 |
2004年 | 67篇 |
2003年 | 59篇 |
2002年 | 45篇 |
2001年 | 38篇 |
2000年 | 31篇 |
1999年 | 17篇 |
1998年 | 10篇 |
1997年 | 14篇 |
1996年 | 7篇 |
1995年 | 10篇 |
1994年 | 9篇 |
1993年 | 7篇 |
1992年 | 13篇 |
1991年 | 13篇 |
1990年 | 11篇 |
1989年 | 16篇 |
1988年 | 6篇 |
1987年 | 9篇 |
1986年 | 8篇 |
1985年 | 8篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 11篇 |
1978年 | 5篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1967年 | 4篇 |
排序方式: 共有2031条查询结果,搜索用时 15 毫秒
1.
A.C. Gonçalves T.D. Matos H.R. Simões-Teixeira M. Pimenta Machado M. Simão Ó.P. Dias M. Andrea G. Fialho H. Caria 《Gene》2014
Low-frequency sensorineural hearing loss (LFSNHL) is an unusual type of HL in which frequencies at 2000 Hz and below are predominantly affected. Most of the families with LFSNHL carry missense mutations in WFS1 gene, coding for wolframin. 相似文献
2.
Fátima H. Vaz Patrícia M. Machado Rita D. Brand?o Cátia T. Laranjeira Joana S. Eugénio Aires H. Fernandes Saudade P. André 《The journal of histochemistry and cytochemistry》2007,55(11):1105-1113
Only 20-25% of families screened for BRCA1/2 mutations are found positive. Because only a positive result is informative, we studied the role of BRCA1/2 immunohistochemistry as an additional method for patient selection. From 53 high-risk-affected probands, 18 (34%) had available paraffin blocks of their tumors and were selected for this study. Mutation screening was done by conformation-sensitive gel electrophoresis and multiplex ligation-dependent probe amplification. For immunohistochemistry, 21 neoplastic specimens (15 breast carcinomas, 5 ovary neoplasms, and 1 rectal adenocarcinoma) were analyzed with BRCA1 (monoclonal antibody, Ab-1, oncogene) and BRCA2 (polyclonal antibody, Ab-2, oncogene) antibodies. Absence of the BRCA1 protein was confirmed in negative tumors by Western blotting. Seven patients were positive for BRCA1/2 mutations: 5 for BRCA1 and 2 for BRCA2. Four out of five positive patients had tumors negative for BRCA1 immunostaining, and the remaining 13 BRCA1-negative patients had positive BRCA1 immunostaining in all tumor samples. Sensitivity to predict for BRCA1 mutation carriers was 80%, and specificity was 100%, with a positive predictive value of 100% and a negative predictive value of 93%. This correlation was statistically significant (p=0.001). No correlation was observed for BRCA2. If larger studies confirm these results, high-risk patients with BRCA1-negative tumors should be screened first for this gene. 相似文献
3.
Filippo Genovese Stefania Ferrari Giambattista Guaitoli Monica Caselli M. Paola Costi Glauco Ponterini 《Protein science : a publication of the Protein Society》2010,19(5):1023-1030
An ad hoc bioconjugation/fluorescence resonance energy transfer (FRET) assay has been designed to spectroscopically monitor the quaternary state of human thymidylate synthase dimeric protein. The approach enables the chemoselective engineering of allosteric residues while preserving the native protein functions through reversible masking of residues within the catalytic site, and is therefore suitable for activity/oligomerization dual assay screenings. It is applied to tag the two subunits of human thymidylate synthase at cysteines 43 and 43′ with an excitation energy donor/acceptor pair. The dimer–monomer equilibrium of the enzyme is then characterized through steady‐state fluorescence determination of the intersubunit resonance energy transfer efficiency. 相似文献
4.
5.
6.
7.
Xu Zhang Wei Zhang Santosh L. Saraf Mehdi Nouraie Jin Han Michel Gowhari Johara Hassan Galina Miasnikova Adelina Sergueeva Sergei Nekhai Rick Kittles Roberto F. Machado Joe G. N. Garcia Mark T. Gladwin Martin H. Steinberg Paola Sebastiani Donald A. McClain Victor R. Gordeuk 《Human genetics》2015,134(8):895-904
8.
9.
10.
D. Dimou A.M. D'Onghia M. Laimer da Camara Machado V. Savino 《Journal of Phytopathology》1994,142(4):258-262
A virus recovered by inoculation of sap from Austrian vines with yellow mosaic symptoms was compared with, and found virtually indistinguishable from, an authentic Hungarian isolate of grapevine chrome mosaic nepovirus. This seems to be the first record of the virus in Austria. 相似文献